Clinical Chemistry
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Clinical Chemistry 26: 156-158, 1980;
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Clinical Chemistry, Vol 26, 156-158, Copyright © 1980 by American Association for Clinical Chemistry

Use of an alumina column in estimating total iron-binding capacity

RT Starr

I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.


The following articles in journals at HighWire Press have cited this article:


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Clin. Chem.Home page
H. Yamanishi, S. Iyama, Y. Yamaguchi, Y. Kanakura, and Y. Iwatani
Total Iron-binding Capacity Calculated from Serum Transferrin Concentration or Serum Iron Concentration and Unsaturated Iron-binding Capacity
Clin. Chem., January 1, 2003; 49(1): 175 - 178.
[Full Text] [PDF]


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Clin. Chem.Home page
G. Siek, J. Lawlor, D. Pelczar, M. Sane, and J. Musto
Direct Serum Total Iron-binding Capacity Assay Suitable for Automated Analyzers
Clin. Chem., January 1, 2002; 48(1): 161 - 166.
[Abstract] [Full Text] [PDF]


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Clin. Chem.Home page
H. Yamanishi, S. Kimura, S. Iyama, Y. Yamaguchi, and T. Yanagihara
Fully automated measurement of total iron-binding capacity in serum
Clin. Chem., December 1, 1997; 43(12): 2413 - 2417.
[Abstract] [Full Text] [PDF]


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Arch Pediatr Adolesc MedHome page
M. Tenenbein and R. W. Yatscoff
The Total Iron-Binding Capacity in Iron Poisoning: Is It Useful?
Arch Pediatr Adolesc Med, April 1, 1991; 145(4): 437 - 439.
[Abstract] [PDF]




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Copyright © 1980 by the American Association for Clinical Chemistry.