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Clinical Chemistry, Vol 26, 156-158, Copyright © 1980 by American Association for Clinical Chemistry
RT Starr
I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.
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M. Tenenbein and R. W. Yatscoff The Total Iron-Binding Capacity in Iron Poisoning: Is It Useful? Arch Pediatr Adolesc Med, April 1, 1991; 145(4): 437 - 439. [Abstract] [PDF] |
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