Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 1450-1453, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fosslien, E.
Right arrow Articles by Nastir, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fosslien, E.
Right arrow Articles by Nastir, A.

Clinical Chemistry, Vol 26, 1450-1453, Copyright © 1980 by American Association for Clinical Chemistry

Continuous-flow zone electrophoresis: experimental demonstration and discussion of potential clinical and research applications

E Fosslien, DN Anderson and A Nastir

We report continuous-flow sample application and continuous electrophoretic separation on a moving cellulose acetate tape. This very simple automated electrophoretic system requires minimum electronic control circuitry. Samples and calibrators are pumped by a peristaltic pump from sample containers onto a continuously moving cellulose acetate tape. Applied samples are interspaced with rinse solution (buffer) and small air segments for sample integrity. Electrophoretic separation, staining, and destaining are all continuous functions performed on the continuously moving tape. The system appears suitable for separation of proteins. Continuous-flow application could be combined with discrete sample application onto the same tape for continuous counter-immunoelectrophoresis; moreover, counter- electrophoresis of two continuously applied proteins, with one applied in a gradient, could potentially produce a system for automated gradient counter-immunoelectrophoresis. It should also be possible to design a simple system for automated immunofixation electrophoresis.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.