Clinical Chemistry
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Clinical Chemistry 26: 1548-1553, 1980;
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Clinical Chemistry, Vol 26, 1548-1553, Copyright © 1980 by American Association for Clinical Chemistry

Comparability of results by simplified methods for measuring serum lipoprotein cholesterol in a pediatric population

SR Srinivasan, TA Foster and GS Berenson

We compared serum lipoprotein cholesterol concentrations in a randomly selected subsample (n = 406) population of children (ages 5 to 17 years), as measured by the heparin-Ca2+ and the heparin-Mn2+ precipitation methods. Statistical analysis showed significant differences between results by these methods, especially in values for VLDL- and HDL-cholesterol (mean difference: VLDL-cholesterol, -61 mg/L; HDL-cholesterol, + 108 mg/L). Lipoprotein electrophoretic data indicated that estimating VLDL-cholesterol from serum triglyceride concentrations invariably resulted in higher values for children with an extremely faint pre-beta-lipoprotein band. Among the HDL- subfractions, 14 to 20% of HDL2 was precipitated by heparin-Mn2+ despite the presence of 80 g of albumin per liter. Heparin affinity chromatography of HDL2 in the presence of Ca2+ and Mn2+ showed an interaction of apolipoprotein E-containing HDL2 subfraction only in conditions involving Mn2+. The heparin-Ca2+ procedure precipitated the equivalent of 2 g of beta- plus pre-beta-lipoprotein cholesterol per liter from serum, with no detectable apolipoprotein B in the supernate. We conclude that these precipitations are affected by cation specificity and by differences in lipoprotein makeup of a given individual or population group.


The following articles in journals at HighWire Press have cited this article:


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 JAMAHome page
D. M. DeLong, E. R. DeLong, P. D. Wood, K. Lippel, and B. M. Rifkind
A Comparison of Methods for the Estimation of Plasma Low- and Very Low-Density Lipoprotein Cholesterol: The Lipid Research Clinics Prevalence Study
JAMA, November 7, 1986; 256(17): 2372 - 2377.
[Abstract] [PDF]

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 Arch Pediatr Adolesc MedHome page
J. L. Cresanta, S. R. Srinivasan, L. S. Webber, and G. S. Berenson
Serum Lipid and Lipoprotein Cholesterol Grids for Cardiovascular Risk Screening of Children
Arch Pediatr Adolesc Med, April 1, 1984; 138(4): 379 - 387.
[Abstract] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.