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Clinical Chemistry 26: 1583-1587, 1980;
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Clinical Chemistry, Vol 26, 1583-1587, Copyright © 1980 by American Association for Clinical Chemistry

Rapid, fully automated radioimmunoassay of prostatic acid phosphatase in serum

S Dass, NL Bowen and KD Bagshawe

Prostatic acid phosphatase was purified from human semen and its purity established by biochemical and immunological criteria. Rabbits were injected with the purified isoenzyme to raise specific antisera. The prostatic acid phosphatase was radiolabeled with 125I by the Chloramine T method. We developed a fully automated double-antibody radiommunosassay for measuring prostatic acid phosphatase in serum from patients with carcinoma of the prostate and from several control groups. The lower detection limit of the radioimmunoassay was 2.0 microgram of prostatic acid phosphatase per litre of serum. Values for most members of the control group was <2.0 microgram/L; patients with metastatic carcinoma of the prostate had values ranging from <2.0 to 300 microgram/L of serum.




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Copyright © 1980 by the American Association for Clinical Chemistry.