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Clinical Chemistry 26: 1729-1732, 1980;
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Clinical Chemistry, Vol 26, 1729-1732, Copyright © 1980 by American Association for Clinical Chemistry

Effect of detergent on kinetic Jaffe-method assay of creatinine

A Bergman and G Ohman

Using isotope dilution-mass spectrometry as reference method, we show that the kinetic Jaffe method for determination of serum creatinine sometimes produces results that are much too high. Such falsely increased values were obtained in about 10% of the sera collected for routine determination of creatinine, and were most common when certain types of collecting tubes were used. The interferences could be overcome by use of detergent in the reaction mixture.




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Copyright © 1980 by the American Association for Clinical Chemistry.