Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 1775-1779, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Demacker, P. N.
Right arrow Articles by Jansen, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demacker, P. N.
Right arrow Articles by Jansen, A. P.

Clinical Chemistry, Vol 26, 1775-1779, Copyright © 1980 by American Association for Clinical Chemistry

A study of the use of polyethylene glycol in estimating cholesterol in high-density lipoprotein

PN Demacker, AG Hijmans, HE Vos-Janssen, A van't Laar and AP Jansen

We studied polyethylene glycol 6000 precipitation of lipoproteins other than high-density lipoproteins, before cholesterol is estimated in the supernate. Other lipoproteins in the supernatant fractions were detected by using rocket immunoelectrophoresis. A polyethylene glycol concentration of 75 g/L in the final mixture appeared to be optimal, and results agreed with those obtained by ultracentrifugation. Differences in serum pH, use of polyethylene glycol from different suppliers, or the presence of ethylenediaminetetraacetate resulted in values that differed significantly (by 40 to 60 mumol/L) from the reference values. Polyethylene glycol did not interfere in four different methods for determination of cholesterol. In combination with an enzymic cholesterol method, the polyethylene glycol method appeared to be very precise, even when lipemic sera (triglycerides up to 5.5 mmol/L) were analyzed that had diminished high-density lipoprotein cholesterol values. We consider this method a method of choice, especially when lipemic sera are tested and enzymic cholesterol analysis is used.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
J. de Graaf, G. M. van der Vleuten, E. ter Avest, G. M. Dallinga-Thie, and A. F. H. Stalenhoef
High Plasma Level of Remnant-Like Particles Cholesterol in Familial Combined Hyperlipidemia
J. Clin. Endocrinol. Metab., April 1, 2007; 92(4): 1269 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
G. M. van der Vleuten, L. J. H. van Tits, M. den Heijer, H. Lemmers, A. F. H. Stalenhoef, and J. de Graaf
Decreased adiponectin levels in familial combined hyperlipidemia patients contribute to the atherogenic lipid profile
J. Lipid Res., November 1, 2005; 46(11): 2398 - 2404.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. J. Veerkamp, J. de Graaf, J. C.M. Hendriks, P. N.M. Demacker, and A. F.H. Stalenhoef
Nomogram to Diagnose Familial Combined Hyperlipidemia on the Basis of Results of a 5-Year Follow-Up Study
Circulation, June 22, 2004; 109(24): 2980 - 2985.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. J. Veerkamp, J. de Graaf, S. J.H. Bredie, J. C.M. Hendriks, P. N.M. Demacker, and A. F.H. Stalenhoef
Diagnosis of Familial Combined Hyperlipidemia Based on Lipid Phenotype Expression in 32 Families: Results of a 5-Year Follow-Up Study
Arterioscler Thromb Vasc Biol, February 1, 2002; 22(2): 274 - 282.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. N. Nanjee, C. J. Cooke, W. L. Olszewski, and N. E. Miller
Lipid and apolipoprotein concentrations in prenodal leg lymph of fasted humans: associations with plasma concentrations in normal subjects, lipoprotein lipase deficiency, and LCAT deficiency
J. Lipid Res., August 1, 2000; 41(8): 1317 - 1327.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
J. A. M. Beentjes, A. van Tol, W. J. Sluiter, and R. P. F. Dullaart
Effect of growth hormone replacement therapy on plasma lecithin:cholesterol acyltransferase and lipid transfer protein activities in growth hormone-deficient adults
J. Lipid Res., June 1, 2000; 41(6): 925 - 932.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
S. C. Riemens, A. Van Tol, W. J. Sluiter, and R. P. F. Dullaart
Acute and chronic effects of a 24-hour intravenous triglyceride emulsion challenge on plasma lecithin: cholesterol acyltransferase, phospholipid transfer protein, and cholesteryl ester transfer protein activities
J. Lipid Res., August 1, 1999; 40(8): 1459 - 1466.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
S. C. Riemens, A. Van Tol, B. K. Stulp, and R. P. F. Dullaart
Influence of insulin sensitivity and the TaqIB cholesteryl ester transfer protein gene polymorphism on plasma lecithin:cholesterol acyltransferase and lipid transfer protein activities and their response to hyperinsulinemia in non-diabetic men
J. Lipid Res., August 1, 1999; 40(8): 1467 - 1474.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. N. Nanjee, J. E. Doran, P. G. Lerch, and N. E. Miller
Acute Effects of Intravenous Infusion of ApoA1/Phosphatidylcholine Discs on Plasma Lipoproteins in Humans
Arterioscler Thromb Vasc Biol, April 1, 1999; 19(4): 979 - 989.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. Cobbaert, P. G.H. Mulder, H. Baadenhuijsen, L. Zwang, C. W. Weykamp, and P. N.M. Demacker
Survey of Total Error of Precipitation and Homogeneous HDL-Cholesterol Methods and Simultaneous Evaluation of Lyophilized Saccharose-containing Candidate Reference Materials for HDL-Cholesterol
Clin. Chem., March 1, 1999; 45(3): 360 - 370.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
P. Zandberg, J. L. M. Peters, P. N. M. Demacker, M. J. Smit, E. G. de Reeder, and D. G. Meuleman
Tibolone Prevents Atherosclerotic Lesion Formation in Cholesterol-Fed, Ovariectomized Rabbits
Arterioscler Thromb Vasc Biol, December 1, 1998; 18(12): 1844 - 1854.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. J. Smilde, F. W. P. J. van den Berkmortel, G. H. J. Boers, H. Wollersheim, T. de Boo, H. van Langen, and A. F. H. Stalenhoef
Carotid and Femoral Artery Wall Thickness and Stiffness in Patients at Risk for Cardiovascular Disease, With Special Emphasis on Hyperhomocysteinemia
Arterioscler Thromb Vasc Biol, December 1, 1998; 18(12): 1958 - 1963.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
G. M. Darling, J. A. Johns, P. I. McCloud, and S. R. Davis
Estrogen and Progestin Compared with Simvastatin for Hypercholesterolemia in Postmenopausal Women
N. Engl. J. Med., August 28, 1997; 337(9): 595 - 601.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S.J.H. Bredie, J. van Drongelen, L.A. Kiemeney, P.N.M. Demacker, T.H. Beaty, and A.F.H. Stalenhoef
Segregation Analysis of Plasma Apolipoprotein B Levels in Familial Combined Hyperlipidemia
Arterioscler Thromb Vasc Biol, May 1, 1997; 17(5): 834 - 840.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
P. N. M. Demacker, M. Hessels, H. Toenhake-Dijkstra, and H. Baadenhuijsen
Precipitation methods for high-density lipoprotein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio
Clin. Chem., April 1, 1997; 43(4): 663 - 668.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. B. de Rijke*, S. J.H. Bredie*, P. N.M. Demacker, J. M. Vogelaar, H. L.M. Hak-Lemmers, and A. F.H. Stalenhoef
The Redox Status of Coenzyme Q10 in Total LDL as an Indicator of In Vivo Oxidative Modification: Studies on Subjects With Familial Combined Hyperlipidemia
Arterioscler Thromb Vasc Biol, January 1, 1997; 17(1): 127 - 133.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M.N. Nanjee, J.R. Crouse, J.M. King, R. Hovorka, S.E. Rees, E.R. Carson, J.-J. Morgenthaler, P. Lerch, and N.E. Miller
Effects of Intravenous Infusion of Lipid-Free Apo A-I in Humans
Arterioscler Thromb Vasc Biol, September 1, 1996; 16(9): 1203 - 1214.
[Abstract] [Full Text]

Home page
 CirculationHome page
A. A. Kroon, W. R.M. Aengevaeren, T. van der Werf, G. J.H. Uijen, J. H.C. Reiber, A. V.G. Bruschke, and A. F.H. Stalenhoef
LDL-Apheresis Atherosclerosis Regression Study (LAARS) : Effect of Aggressive Versus Conventional Lipid Lowering Treatment on Coronary Atherosclerosis
Circulation, May 15, 1996; 93(10): 1826 - 1835.
[Abstract] [Full Text]

Home page
 BMJHome page
Randomised comparison of oestrogen versus oestrogen plus progestogen hormone replacement therapy in women with hysterectomy
BMJ, February 24, 1996; 312(7029): 473 - 478.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. A. Kuivenhoven, A. F.H. Stalenhoef, J. S. Hill, P. N.M. Demacker, A. Errami, J. J.P. Kastelein, and P. H. Pritchard
Two Novel Molecular Defects in the LCAT Gene Are Associated With Fish Eye Disease
Arterioscler Thromb Vasc Biol, February 1, 1996; 16(2): 294 - 303.
[Abstract] [Full Text]

Home page
 Arch Intern MedHome page
J. C. Defesche, K. L. Pricker, M. R. Hayden, B. E. van der Ende, and J. J. P. Kastelein
Familial Defective Apolipoprotein B-100 Is Clinically Indistinguishable From Familial Hypercholesterolemia
Arch Intern Med, October 25, 1993; 153(20): 2349 - 2356.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.