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Clinical Chemistry 26: 1791-1795, 1980;
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Clinical Chemistry, Vol 26, 1791-1795, Copyright © 1980 by American Association for Clinical Chemistry

Assay for trimethoprim in serum and urine by means of ion-pair chromatography

ID Watson, A Shenkin, SJ McIntosh and HN Cohen

We describe a rapid, precise, and reliable procedure for assay of trimethoprim in serum and urine by ion-pair chromatography. Trimethoprim concentrations in urine are determined by an externally standardized, direct-injection procedure; assay in serum involves a simple preliminary extraction and internal standardization. The assays are suitable for pharmacokinetic studies and have been applied to determination of trimethoprim concentrations in serum and urine during therapy with Co-trimoxazole, a sulfonamide/trimethoprim preparation.




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Copyright © 1980 by the American Association for Clinical Chemistry.