Clinical Chemistry
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Clinical Chemistry 26: 1871-1873, 1980;
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Clinical Chemistry, Vol 26, 1871-1873, Copyright © 1980 by American Association for Clinical Chemistry

Measurement of ultrafiltrable calcium in serum with use of the "Worthington Ultrafree Anticonvulsant Drug Filter"

JH Eckfeldt and DF Koehler

We describe a method for ultrafiltrable calcium involving the use of Worthington Ultrafree Anticonvulsant Drug Filters. When measured at 37 degrees C, with mineral oil covering the sample to prevent loss of CO2, values for ultrafiltrable calcium correlated reasonably well (r = 0.91) with those for ionized calcium as measured with an ion-selective electrode. All patients' samples with significantly high or low values for ionized calcium were identified by the ultrafiltration method, including one specimen for which the McLean-Hastings protein correction could not explain the discrepancy between ionized and total calcium. The method requires about 2 mL of serum, yields about 100 microL of protein-free ultrafiltrate, and with it any laboratory with a semi- micro calcium method can measure ultrafiltrable calcium.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Cochran, B. Rumbelow, and G. Allen
The Relation between the Ultrafiltrable Calcium Fraction and Blood pH and Concentrations of Total Plasma Calcium, Albumin, and Globulin
Clin. Chem., July 1, 1998; 44(7): 1559 - 1562.
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Copyright © 1980 by the American Association for Clinical Chemistry.