|
|
||||||||
Clinical Chemistry, Vol 26, 1899-1902, Copyright © 1980 by American Association for Clinical Chemistry
O Lantto, I Bjorkhem, R Blomstrand and A Kallner
We used four RIA kits for determination of plasma cortisol to assay 12 plasma samples in four laboratories, and compared the results with those obtained with a highly accurate method based on isotope dilution/mass spectrometry. All kits (Farmos, NEN, Sorin, and Clinical Assays) showed 10-50% deviation from the comparison method in the normal range (200-700 nmol/L). The inter- and intralaboratory imprecision (CV) ranged between 8-27% and 5-11%, respectively. Possible explanations for this low accuracy are discussed. Retention of an intact biological matrix in the calibration standards seems to be one prerequisite for accuracy.
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |