Clinical Chemistry
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Clinical Chemistry 26: 205-208, 1980;
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Clinical Chemistry, Vol 26, 205-208, Copyright © 1980 by American Association for Clinical Chemistry

Simultaneous liquid-chromatographic determination of zinc protoporphyrin IX, protoporphyrin IX, and coproporphyrin in whole blood

GR Gotelli, JH Wall, PM Kabra and LJ Marton

We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-to-day precision (CV) from 5 to 12%. Uroporphyrin, though not studied in detail, can also be detected by this method.




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Copyright © 1980 by the American Association for Clinical Chemistry.