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Clinical Chemistry 26: 277-281, 1980;
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Clinical Chemistry, Vol 26, 277-281, Copyright © 1980 by American Association for Clinical Chemistry

Amniotic fluid phosphatidylglycerol and phosphatidylinositol separated by stepwise-development thin-layer chromatography

M Mitnick, B DeMarco and JM Gibbons

We describe a stepwise-development thin-layer-chromatographic technique for separating phosphatidylglycerol and phosphatidylinositol in samples of amniotic fluid. Plates are prepared from silica gel G slurried in a 50 g/L ammonium sulfate solution. Phospholipid phosphorus determination is not needed. Phospholipid phosphorus determination is not needed. Phosphatidylglycerol and phosphatidylinositol, measured densitometrically, are expressed as ratios to the sphingomyelin present. These ratios are correlated with the lecithin/sphingomyelin ratio and age of gestation in normal and abnormal pregnancies.


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