Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 291-294, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soldin, S. J.
Right arrow Articles by Hill, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soldin, S. J.
Right arrow Articles by Hill, J. G.

Clinical Chemistry, Vol 26, 291-294, Copyright © 1980 by American Association for Clinical Chemistry

Simultaneous liquid-chromatographic analysis for 4-hydroxy-3- methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine

SJ Soldin and JG Hill

We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5- mL aliquot of urine is adjusted to pH 6.1 and subjected to anion- exchange chromatography (Dowex AG 1-X4, 1.8 X 0.7 cm column). The two analytes are eluted from the column with 10 mL of 3 mol/L sodium chloride, and 2 microL of the eluate, injected directly into the liquid chromatograph, is chromatographed at 40 degrees C. This column contains mu Bondapak C18, and the mobile phase is 100 mmol/L potassium phosphate buffer, pH 6.7, containing 0.5 mmol of tetrabutylammonium phosphate per liter. Detector sensitivity is 50 nA full scale. Analytical recoveries of I and II are 80--84% and 48--49%, respectively; the coefficients of variation for various concentrations in urine are about 10%.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Pharmacol. Exp. Ther.Home page
J. Riba, M. Valle, G. Urbano, M. Yritia, A. Morte, and M. J. Barbanoj
Human Pharmacology of Ayahuasca: Subjective and Cardiovascular Effects, Monoamine Metabolite Excretion, and Pharmacokinetics
J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 73 - 83.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
W. D. Graf, A. S. Unis, C. M. Yates, S. Sulzbacher, M. B. Dinulos, R. M. Jack, K. A. Dugaw, M. N. Paddock, and W. W. Parson
Catecholamines in patients with 22q11.2 deletion syndrome and the low-activity COMT polymorphism
Neurology, August 14, 2001; 57(3): 410 - 416.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.