Clinical Chemistry
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Clinical Chemistry 26: 297-300, 1980;
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Clinical Chemistry, Vol 26, 297-300, Copyright © 1980 by American Association for Clinical Chemistry

Binding of enzyme--IgG complexes in human serum to Protein-A Sepharose CL-4B

K Bauer, PM Bayer, E Deutsch and F Gabl

We describe a simple method for detecting enzyme--immunoglobulin G (IgG) complexes in human serum. Protein-A Sepharose CL-4B binds IgG and therefore also the enzyme--IgG complexes, which can then be separated easily from the serum by centrifugation. We demonstrate this separation in two patients, one with a complex of IgG and creatine kinase (EC 2.7.3.2) BB isoenzyme, the other with an IgG--alkaline phosphatase (EC 3.1.3.1) complex. Both patients had unexplainably high activities of the respective enzymes in their serum. The method we propose should be a useful, simple, routine method of detection in cases where IgG-- enzyme complexes are suspected.


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 Arch Intern MedHome page
P. L. Wolf
Lactate Dehydrogenase--6: A Biochemical Sign of Serious Hepatic Circulatory Disturbance
Arch Intern Med, August 1, 1985; 145(8): 1396 - 1397.
[Abstract] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.