Clinical Chemistry
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Clinical Chemistry 26: 312-313, 1980;
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Clinical Chemistry, Vol 26, 312-313, Copyright © 1980 by American Association for Clinical Chemistry

Improved protein-binding radioassay for plasma cortisol

H Novacenko, F Halpern and EJ Sachar

We describe an improved competitive protein-binding radioassay for total plasma cortisol, which permits rapid analysis of many samples. Plasma, 0.1 mL, is directly applied to a Whatman 3MM filter-paper strip, which is extracted once with dichloromethane. The extract is evaporated and the residue is assayed directly by the micro-method of Murphy (J. Clin. Endocrinol. Metab. 27: 973, 1967). Comparison with the original Murphy method (ethanol extraction) yielded excellent agreement (r = 0.988). One hundred samples, in duplicate, can easily be assayed at a time.


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J. Puig-Antich, R. Dahl, N. Ryan, H. Novacenko, D. Goetz, R. Goetz, J. Twomey, and T. Klepper
Cortisol Secretion in Prepubertal Children With Major Depressive Disorder: Episode and Recovery
Arch Gen Psychiatry, September 1, 1989; 46(9): 801 - 809.
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E. Hollander, M. R. Liebowitz, J. M. Gorman, B. Cohen, A. Fyer, and D. F. Klein
Cortisol and Sodium Lactate--Induced Panic
Arch Gen Psychiatry, February 1, 1989; 46(2): 135 - 140.
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U. Halbreich, G. M. Asnis, R. Shindledecker, B. Zumoff, and R. S. Nathan
Cortisol Secretion in Endogenous Depression: I. Basal Plasma Levels
Arch Gen Psychiatry, September 1, 1985; 42(9): 904 - 908.
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E. J. Sachar, U. Halbreich, G. M. Asnis, R. S. Nathan, F. S. Halpern, and L. Ostrow
Paradoxical Cortisol Responses to Dextroamphetamine in Endogenous Depression
Arch Gen Psychiatry, October 1, 1981; 38(10): 1113 - 1117.
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Copyright © 1980 by the American Association for Clinical Chemistry.