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Clinical Chemistry 26: 441-443, 1980;
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Clinical Chemistry, Vol 26, 441-443, Copyright © 1980 by American Association for Clinical Chemistry

Mannitol estimation in biological fluids by gas-liquid chromatography of trimethylsilyl derivatives

MF Laker and JN Mount

We describe a procedure for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (alpha-methylglucose), deproteinized if necessary, desalted, and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60 degrees C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190 degrees C, and quantitated by peak- height measurement. The technique is linear, accurate, precise, sensitive, and free from interference. It has been used to measure mannitol in plasma, urine, and bile.


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R. Marsilio, L. D'Antiga, L. Zancan, N. Dussini, and F. Zacchello
Simultaneous HPLC determination with light-scattering detection of lactulose and mannitol in studies of intestinal permeability in pediatrics
Clin. Chem., August 1, 1998; 44(8): 1685 - 1691.
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Copyright © 1980 by the American Association for Clinical Chemistry.