Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 444-450, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mason, R. S.
Right arrow Articles by Posen, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mason, R. S.
Right arrow Articles by Posen, S.

Clinical Chemistry, Vol 26, 444-450, Copyright © 1980 by American Association for Clinical Chemistry

A simplified assay for dihydroxylated vitamin D metabolites in human serum: application to hyper- and hypovitaminosis D

RS Mason, D Lissner, HS Grunstein and S Posen

We describe a simplified assay for 24,25-and 1.25-dihydroxyvitamin D in human serum. It involves two preparative steps, and normal chick intestine is used in preparing cytosol-binding protein. Our results for 24,25-dihydroxyvitamin D include a reference interval of 2.9--16 nmol/L (1.2--6.7 microgram/L), a mean of 6.7 nmol/L (2.8 microgram/L), an intra-assay CV of 11%, and an interassay CV of 22%. For 1,25- dihydroxyvitamin D, these data were 29--168 pmol/L (12--70 ng/L), 86 pmol/L (36 ng/L), 12%, and 22%, respectively. In hypoparathyroid patients with vitamin D intoxication, mean concentrations of 25- hydroxyvitamin D and 24,25-dihydroxyvitamin D in serum were significantly above normal; the 1,25-dihydroxyvitamin D concentrations were significantly below normal. Patients with malabsorption and/or post-gastrectomy states had significantly subnormal values for both 25- hydroxyvitamin D and 24,25-dihydroxyvitamin D in serum, and there was a significantly negative correlation between each of these biochemical values and the severity of osteomalacia. We also discuss cost effectiveness of assaying vitamin D metabolites in human serum.


The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
X. Du, H. Greenfield, D. R Fraser, K. Ge, A. Trube, and Y. Wang
Vitamin D deficiency and associated factors in adolescent girls in Beijing
Am. J. Clinical Nutrition, October 1, 2001; 74(4): 494 - 500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
R. Vieth
Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety
Am. J. Clinical Nutrition, May 1, 1999; 69(5): 842 - 856.
[Abstract] [Full Text] [PDF]

Home page
 ANN INTERN MEDHome page
R. S. MASON, T. FRANKEL, Y.-L. CHAN, D. LISSNER, and S. POSEN
Vitamin D Conversion by Sarcoid Lymph Node Homogenate
Ann Intern Med, January 1, 1984; 100(1): 59 - 61.
[Abstract] [PDF]

Home page
 Arch Pediatr Adolesc MedHome page
R. S. Mason, P. G. Rohl, D. Lissner, and S. Posen
Vitamin D Metabolism in Hypophosphatemic Rickets
Arch Pediatr Adolesc Med, October 1, 1982; 136(10): 909 - 913.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.