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Clinical Chemistry 26: 451-456, 1980;
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Clinical Chemistry, Vol 26, 451-456, Copyright © 1980 by American Association for Clinical Chemistry

Alkaline phosphatase of high and low molecular mass in human serum and bile: a comparative study of kinetic properties

PM Crofton and AF Smith

We studied the kinetic properties of high- and low-molecular-mass forms of alkaline phosphatase purified from serum and bile, to clarify their interrelationships. They were found to share virtually identical kinetic properties, and to obey the same general kinetics as the liver- derived isoenzyme from serum and the low-molecular-mass isoenzyme from bile with regard to optimum conditions of assay, activation by magnesium ions, inhibition by L-homoarginine, inhibition by nickel and zinc ions, and inactivation by urea. Most of the characteristics such as Km (at low magnesium ion concentrations), Ki for L-homoarginine, and half-life for urea inactivation, were closely similar for low- and high- molecular-mass alkaline phosphatase. We conclude that these forms of alkaline phosphatase in plasma and bile are closely related. We discuss the possible nature of this relationship.




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Copyright © 1980 by the American Association for Clinical Chemistry.