Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 26: 508-510, 1980;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Connolly, B. M.
Right arrow Articles by Goodman, H. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Connolly, B. M.
Right arrow Articles by Goodman, H. O.

Clinical Chemistry, Vol 26, 508-510, Copyright © 1980 by American Association for Clinical Chemistry

Potential sources of errors in cation-exchange chromatographic measurement of plasma taurine

BM Connolly and HO Goodman

We examined the potential sources of error in automated cation-exchange chromatographic quantitation of plasma taurine, both in sample preparation and in the analysis. Principal sources of error include: use of serum instead of plasma, which produces gross overestimates; use of tripotassium ethylenediaminetetraacetate (EDTA) as anticoagulant in systems involving ninhydrin detection (a ninhydrin-positive contaminant of EDTA emerges coincident with taurine); contamination with platelets; and placing volumes exceeding 20 microL on the cartridge used in the Technicon TSM Amino Acid Analyzer. We arrived at a simple technique in which we use EDTA as anticoagulant, micropore filtration to produce platelet-free plasma, and o-phthalaldehyde as the detection reagent for the sensitivity required to measure accurately the low concentration of taurine in plasma.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the American Association for Clinical Chemistry.