Clinical Chemistry
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Clinical Chemistry 26: 551-554, 1980;
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Clinical Chemistry, Vol 26, 551-554, Copyright © 1980 by American Association for Clinical Chemistry

Kinetic serum creatinine assays I. The role of various factors in determining specificity

LD Bowers

We studied the specificity of kinetic methods of analysis, with emphasis on creatinine determination. The error contributed by an interferent that reacts with the analytical reagent and absorbs at the wavelength of analysis is shown to be related to the ratio of the equilibrium absorbances of the analyte and interferent, the ratio of the rate constants, the extent of the analytical reaction, and the duration of the measurement. To assess the potential diminution of the interference with kinetic methods, we determined the rate constants of several alpha-keto acids in the Jaffe reaction. Evaluation of the advantages of various measuring techniques with respect to specificity showed the main factors to be the extent of the analytical reaction and the relative value of the rate constants. Total specificity for creatine appears to be unattainable with currently used kinetic techniques.


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