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Clinical Chemistry 26: 724-729, 1980;
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Clinical Chemistry, Vol 26, 724-729, Copyright © 1980 by American Association for Clinical Chemistry

High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material

GN Bowers Jr, RB McComb, RG Christensen and R Schaffer

We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper molar absorptivity of 4-nitrophenol under final reaction conditions, particularly for measuring alkaline phosphatase activity in human serum. Some lots of 4-nitrophenol available commercially met these specifications, but several did not. The latter can be purified to meet our specifications by recrystallization or sublimation. The molar absorptivity of 4-nitrophenol (35 mumol/L) IN 10 mmol/L NaOH at 25 degrees C at 401 nm is 18380 +/- 90 L.mol-1.cm-1.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
K. Lorentz
Routine {alpha}-Amylase Assay Using Protected 4-Nitrophenyl-1,4-{alpha}-D-maltoheptaoside and a Novel {alpha}-Glucosidase
Clin. Chem., May 1, 2000; 46(5): 644 - 649.
[Abstract] [Full Text] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.