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Clinical Chemistry 26: 745-749, 1980;
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Clinical Chemistry, Vol 26, 745-749, Copyright © 1980 by American Association for Clinical Chemistry

Enzyme-linked immunoassay for placental lactogen in human serum

DJ MacDonald, KM Nicol, A Belfield, MM Shah and DS Mack

We describe an enzyme-linked immunoassay for measuring human placental lactogen (HPL) in serum. After suitable dilution, sera are pipetted into the wells of polyvinyl microtitre plates previously coated with anti-HPL. After incubation the sera are decanted, and replaced by a conjugate of horseradish peroxidase and anti-HPL. An amount of enzyme proportional to the concentration of HPL in the serum is bound to the wells via an antibody--HPL--antibody bridge. Peroxidase activity is measured by oxidation of o-phenylenediamine, the resulting color being related to the concentration of HPL. Results correlate well with those by radioimmunoassay (r = 0.96). Our between-assay coefficient of variation was 13%; no discernible effect of protein was observed. This 4-h assay can be used to monitor placental function in pregnancy.




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Copyright © 1980 by the American Association for Clinical Chemistry.