Clinical Chemistry
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Clinical Chemistry 26: 827-830, 1980;
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Clinical Chemistry, Vol 26, 827-830, Copyright © 1980 by American Association for Clinical Chemistry

Spectrophotometric determination of urinary oxalate with oxalate oxidase prepared from moss

MF Laker, AF Hofmann and BJ Meeuse

A novel spectrophotometric enzymic procedure for estimating oxalic acid in urine is described. Oxalate oxidase, prepared from moss species, converts oxalic acid to hydrogen peroxide and carbon dioxide. Hydrogen peroxide is determined enzymatically with horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone with N,N- dimethylaniline; the resulting indamine due is determined spectrophotometrically at 595 nm. Interfering substances are removed by adsorption to ion-exchange resins and oxidation with charcoal, thus avoiding oxalate recovery problems accompanying oxalate isolation. The procedure is rapid, sensitive, linear, and precise. Results agreed well with those obtained with a widely used chemical technique.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
P. M. Ladwig, R. R. Liedtke, T. S. Larson, and J. C. Lieske
Sensitive Spectrophotometric Assay for Plasma Oxalate
Clin. Chem., December 1, 2005; 51(12): 2377 - 2380.
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C. S. Pundir and C. S. Satyapal
Determination of Urinary Oxalate with Cl- and NO-3 Insensitive Oxalate Oxidase Purified from Sorghum Leaf
Clin. Chem., June 1, 1998; 44(6): 1364 - 1365.
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