Clinical Chemistry
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Clinical Chemistry 26: 831-834, 1980;
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Clinical Chemistry, Vol 26, 831-834, Copyright © 1980 by American Association for Clinical Chemistry

Improved serum-initiated aspartate aminotransferase assay by inhibition of lactate dehydrogenase with oxamate

V Lustig and LW Redman

We describe a serum-inititated aspartate aminotransferase (EC 2.6.1.1) assay that obviates the need for added lactate dehydrogenase (EC 1.1.1.27) in the reagent system. Interference from the oxidation of NADH by endogeneous lactate dehydrogenase is eliminated by adding sodium oxamate, a competitive inhibitor of the enzyme. The advantages of oxamate inhibition over lactate dehydrogenase addition are a shorter preincubation period, an increase in the linear range from less than 600 to more than 1400 U/L, no interference from above-normal concentrations of ketoacids, and elimination of bias between serum- initiated and the standard oxoglutarate-initiated assays for aspartate aminotransferase.




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Copyright © 1980 by the American Association for Clinical Chemistry.