Clinical Chemistry
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Clinical Chemistry 26: 907-909, 1980;
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Clinical Chemistry, Vol 26, 907-909, Copyright © 1980 by American Association for Clinical Chemistry

Liquid-chromatographic assay of urinary 5-hydroxy-3-indoleacetic acid, with electrochemical detection

ZK Shihabi and J Scaro

After extraction with two organic solvent mixtures, urinary 5-hydroxy-3- indoleacetic acid can be assayed by "high-performance" liquid chromatography on a reversed-phase column, with electrochemical detection. Compared to the nitrosonaphthol method (J. Biol. Chem. 216: 499, 1955), this method is more specific for detection of patients with carcinoid tumors.




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Copyright © 1980 by the American Association for Clinical Chemistry.