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Clinical Chemistry 26: 1140-1143, 1980;
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Clinical Chemistry, Vol 26, 1140-1143, Copyright © 1980 by American Association for Clinical Chemistry

Radioimmunoassay of thyrotropin and thyroxine in dried blood by use of Staphylococcus aureus containing "protein A."

ML Mitchell, JJ McKenna and J McIver

We report the use of Staphylococcus aureus rich in protein A as an immunoadsorbent in the radioimmunoassay of thyrotropin and thyroxine in blood collected from newborn infants and dried on filter paper. The procedures for thyrotropin and thyroxine, which differ mainly with respect to concentration and volume of the Staphylococcus aureus suspension, are reliable, rapid, and technically simple. The ease of this method, coupled with its dependability, offers certain advantages over some of the commonly used techniques of separation, and therefore it provides a unique approach to measurement of these analytes in laboratory screening programs for neonatal hypothyroidism.




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