Clinical Chemistry
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Clinical Chemistry 26: 1173-1177, 1980;
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Clinical Chemistry, Vol 26, 1173-1177, Copyright © 1980 by American Association for Clinical Chemistry

Use of the conjugated polyene fatty acid, parinaric acid, in assaying fatty acids in serum or plasma

CB Berde, JA Kerner and JD Johnson

We describe a method for rapidly assaying fatty acid concentrations in plasma or serum. This method is particularly appropriate for monitoring increased concentrations of fatty acids that may be present during intravenous lipid infusion, especially in neonates. Binding cisparinaric acid (9, 11, 13, 15-cis-trans-trans-cis-octadecatetraenoic acid) to albumin shifts the absorption spectrum of the acid. If fatty acids are present in serum, they will compete with parinaric acid for binding, changing the extent of this absorption shift. The measurement requires a spectrophotometer, 30 microL of serum or plasma, and knowledge of the sample's albumin concentration.


The following articles in journals at HighWire Press have cited this article:


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 JPEN J Parenter Enteral NutrHome page
A. D'Harlingue, A. O. Hopper, D. K. Stevenson, S. M. Shahin, and J. A. Kerner JR.
Limited Value of Nephelometry in Monitoring the Administration of Intravenous Fat in Neonates
JPEN J Parenter Enteral Nutr, January 1, 1983; 7(1): 55 - 58.
[Abstract] [PDF]

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 Arch SurgHome page
S. Lanza-Jacoby, S. C. Lansey, M. P. Cleary, and F. E. Rosato
Alterations in Lipogenic Enzymes and Lipoprotein Lipase Activity During Gram-Negative Sepsis in the Rat
Arch Surg, February 1, 1982; 117(2): 144 - 147.
[Abstract] [PDF]

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 JPEN J Parenter Enteral NutrHome page
J. A. Kerner JR., C. Cassani, R. Hurwitz, and C. B. Berde
Monitoring Intravenous Fat Emulsions in Neonates with the Fatty Acid/Serum Albumin Molar Ratio
JPEN J Parenter Enteral Nutr, November 1, 1981; 5(6): 517 - 518.
[Abstract] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.