Clinical Chemistry
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Clinical Chemistry 26: 1255-1260, 1980;
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Clinical Chemistry, Vol 26, 1255-1260, Copyright © 1980 by American Association for Clinical Chemistry

Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay

VC Tsang, BC Wilson and SE Maddison

We describe a single-cuvette enzyme-linked immunosorbent assay (ELISA) based on enzymic rate kinetics. This kinetic assay can yield linear quantitative data on immunoglobin concentrations. Optimum assay conditions, component concentrations, reaction intervals, and pH are described. Assay linearity and sensitivity are demonstrated in systems involving immunoglobulin G and antibodies to it, and with pooled sera from monkeys infected with Schistosoma mansoni.




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Copyright © 1980 by the American Association for Clinical Chemistry.