Clinical Chemistry
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Clinical Chemistry 26: 1278-1280, 1980;
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Clinical Chemistry, Vol 26, 1278-1280, Copyright © 1980 by American Association for Clinical Chemistry

Kinetic ethylene glycol assay with use of yeast alcohol dehydrogenase

JH Eckfeldt and RT Light

We describe a rapid, accurate, and precise two-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100 degrees C. We believe that the method can be readily adaptable to any narrow-bandwidth, stable, temperature- controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected.


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R. T. Light, K. M. Nelson, and J. H. Eckfeldt
Ethylene Glycol Poisoning
JAMA, October 16, 1981; 246(16): 1769 - 1769.
[Abstract] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.