Clinical Chemistry
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Clinical Chemistry 26: 1301-1303, 1980;
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Clinical Chemistry, Vol 26, 1301-1303, Copyright © 1980 by American Association for Clinical Chemistry

Separation of serum prednisolone and prednisolone-21-hemisuccinate by extraction and their concurrent determination by radioimmunoassay

K Yanagibashi, A Mizuchi, H Yotsumoto and Y Miyachi

We describe a simple, sensitive, and reliable radioimmunoassay for prednisolone and prednisolone-21-hemisuccinate in serum. The antiserum produced in rabbits to prednisolone-21-hemisuccinate/bovine serum albumin was specific for prednisolone and prednisolone-21- hemisuccinate. A simple dichloromethane extraction permitted the separation of prednisolone from prednisolone-21-hemisuccinate in the serum samples. Interference by cortisol, although not insignificant, is minimized in this assay. We used the method to measure prednisolone and prednisolone-21-hemisuccinate concentrations after a bolus injection of prednisolone-21-hemisuccinate into human beings and mice.




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Copyright © 1980 by the American Association for Clinical Chemistry.