Clinical Chemistry
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Clinical Chemistry 26: 1317-1322, 1980;
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Clinical Chemistry, Vol 26, 1317-1322, Copyright © 1980 by American Association for Clinical Chemistry

Two-dimensional gel electrophoresis of cerebrospinal fluid proteins

D Goldman, CR Merril and MH Ebert

Two-dimensional electrophoresis, with isoelectric focusing in the first dimension and sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the second, has been adapted for the high-resolution analysis of cerebrospinal fluid proteins. Proteins were detected with a new, highly sensitive silver stain that made visible more than 300 polypeptides from 60 microL of spinal fluid, in highly reproducible patterns. We have mapped these patterns, noting difference between the proteins observed in spinal fluid and plasma, and have prepared a partial map of cerebrospinal fluid proteins.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Cancer Res.Home page
F. W. Khwaja, J. S. Duke-Cohan, D. J. Brat, and E. G. Van Meir
Attractin Is Elevated in the Cerebrospinal Fluid of Patients with Malignant Astrocytoma and Mediates Glioma Cell Migration.
Clin. Cancer Res., November 1, 2006; 12(21): 6331 - 6336.
[Abstract] [Full Text] [PDF]

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C. Merril, D Goldman, S. Sedman, and M. Ebert
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
Science, March 27, 1981; 211(4489): 1437 - 1438.
[Abstract] [PDF]


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Copyright © 1980 by the American Association for Clinical Chemistry.