Clinical Chemistry
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Clinical Chemistry 26: 1348-1350, 1980;
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Clinical Chemistry, Vol 26, 1348-1350, Copyright © 1980 by American Association for Clinical Chemistry

Liquid-chromatographic measurement of urinary monosaccharides

M D'Amboise, T Hanai and D Noel

We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D- fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.




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Copyright © 1980 by the American Association for Clinical Chemistry.