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Clinical Chemistry 27: 113-115, 1981;
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Clinical Chemistry, Vol 27, 113-115, Copyright © 1981 by American Association for Clinical Chemistry

Gas-chromatographic assay for thiopental in plasma, with use of a nitrogen-specific detector

D Jung, M Mayersohn and D Perrier

An accurate, sensitive, and specific gas-liquid-chromatographic procedure is described for determining concentrations of thiopental in human plasma. After a double extraction of 0.2 or 1.0 mL of plasma containing phenobarbital as an internal standard, thiopental and the internal standard are derivatized in a polar non-aqueous solvent system with iodomethane. The reaction mixture is then evaporated, the residue reconstituted with ethyl acetate, and 20 microL injected into a 3% OV- 17 column of a gas chromatograph equipped with a nitrogen-phosphorus detector. Linearity and reproducibility over the concentration range 25 microgram/L to 10 mg/L in plasma are excellent. The sensitivity and wide range of linearity exhibited by this method permit thorough characterization of the disposition of thiopental after the usual induction doses of 3-4 mg/kg of body weight.




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Copyright © 1981 by the American Association for Clinical Chemistry.