Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 27: 144-146, 1981;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robertson, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robertson, W. S.

Clinical Chemistry, Vol 27, 144-146, Copyright © 1981 by American Association for Clinical Chemistry

Optimizing determination of plasma albumin by the bromcresol green dye- binding method

WS Robertson

Some modifications of the conditions of the reaction between plasma and bromcresol green have led to an improved method for determination of plasma albumin with the Vickers M300 multichannel analyzer. Dye concentration and reaction time are the factors principally influencing method specificity, but variable protein composition of human plasma also affects it, so that optimal reaction conditions vary from specimen to specimen. Thus a compromise must be reached such that the best conditions for determining plasma albumin over a range of different protein concentrations are achieved. In the proposed method for the Vickers M300 a reaction time of 12 s (the minimum possible) is used. Comparison with "rocket" immunoelectrophoresis gave the following linear regression: y = 10 + 0.79 x (n = 91; r = 0.96).


The following articles in journals at HighWire Press have cited this article:


Home page
 Nutr Clin PractHome page
G. L. Brackeen, J. S. Dover, and C. L. Long
Serum Albumin: Differences in Assay Specificity
Nutr Clin Pract, December 1, 1989; 4(6): 203 - 205.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Association for Clinical Chemistry.