Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 27: 94-99, 1981;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schellekens, A. P.
Right arrow Articles by van Groenestein, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schellekens, A. P.
Right arrow Articles by van Groenestein, T.

Clinical Chemistry, Vol 27, 94-99, Copyright © 1981 by American Association for Clinical Chemistry

Sources of variation in the column-chromatographic determination of glycohemoglobin (HbA1)

AP Schellekens, GT Sanders, W Thornton and T van Groenestein

We studied the influence of many variables on the cation-exchange chromatographic determination of glycohemoglobin was minicolumns (HbA1) and with macrocolumns (HbA1a+b and HbA1c). Minicolumns are most sensitive to buffer composition. The best conditions are pH 6.78 and sodium, potassium, and phosphate concentrations of 54.8, and 36 mmol/L, respectively. In fact, there is no clear cut-off point for elution. A cyanide concentration of 1.5 mmol/L has no negative effects. For macrocolumns, the influence of temperature is demonstrated; 20 degrees C is optimal. A higher temperature caused results for HbA1 to be higher. For minicolumns, the temperature effect corresponds to 1% HbA1 per 1 degrees C, necessitating rigorous temperature control. The amount of sample applied also influences the results. Therefore a constant column load, chosen between 1.3 and 2.0 mg of Hb permilliliter of resin, is advised. Heparinized blood can be kept for three days at 4 degrees C; hemolyzed samples are best stored at -70 degrees C.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Association for Clinical Chemistry.