Clinical Chemistry
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Clinical Chemistry 27: 1651-1654, 1981;
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Clinical Chemistry, Vol 27, 1651-1654, Copyright © 1981 by American Association for Clinical Chemistry

A candidate reference method for determination of total protein in serum. II. Test for transferability

BT Doumas, DD Bayse, K Borner, RJ Carter, F Elevitch, CC Garber, RA Graby, LL Hause, A Mather, T Peters Jr, RN Rand, DJ Reeder, SM Russell, R Schaffer and JO Westgard

The transferability of the candidate Reference Method for total serum protein was tested in eight laboratories in the United States and Europe. National Bureau of Standards SRM 927 (bovine serum albumin) was used in each analytical run as the calibration standard. The mean absorptivity value obtained for this material was 0.2983 L g-1 cm-1. Four serum pools prepared at the Centers for Disease Control were analyzed on each of 15 days. Within-run variation of the protein values (expressed as CV) in the eight laboratories ranged from 0.1 to 2.5% and day-to-day (total) variation in six of the laboratories ranged from 0.4 to 1%.




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Copyright © 1981 by the American Association for Clinical Chemistry.