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Clinical Chemistry 27: 1739-1741, 1981;
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Clinical Chemistry, Vol 27, 1739-1741, Copyright © 1981 by American Association for Clinical Chemistry

Evaluation of chronic granulomatous disease by a chemiluminescence assay of microliter quantities of whole blood

LR DeChatelet and PS Shirley

In our modification of a chemiluminescence assay to detect chronic granulomatous disease of childhood, whole blood is used and dark- adaptation is not required. This improvement was made possible by the use of luminol as an amplification device and of phorbol myristate acetate as a stimulant. Using this simplified assay, we can assess neutrophil function with 25-100 microL of whole blood, permitting simple evaluation even in very young pediatric patients.


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