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Clinical Chemistry 27: 1888-1891, 1981;
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Clinical Chemistry, Vol 27, 1888-1891, Copyright © 1981 by American Association for Clinical Chemistry

Continuous-flow enzymic determination of creatine in urine

M Yasuhara, S Fujita, I Furukawa, K Arisue, K Kohda and C Hayashi

A new enzymic method is described for the determination of creatine in urine by continuous-flow analysis. The measurement is accomplished by transforming creatine to formaldehyde in reactions catalyzed by creatinase (creatine amidinohydrolase) and sarcosine dehydrogenase. The formaldehyde is reacted with 4-amino-3-hydrazino-5-mercapto-1,2,4- triazole to form a purple product, which is measured colorimetrically. The method gives a linear standard curve for creatine concentrations up to 100 mg/L. Precision and analytical recovery are excellent, and results correlate well with those by the more-difficult Folin method, which currently is most often used for this analysis.




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Copyright © 1981 by the American Association for Clinical Chemistry.