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Clinical Chemistry 27: 1892-1895, 1981;
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Clinical Chemistry, Vol 27, 1892-1895, Copyright © 1981 by American Association for Clinical Chemistry

A model for validation of radioimmunoassay kit reagents: measurement of follitropin and lutropin in blood and urine

SJ Santner, RJ Santen, HE Kulin and LM Demers

We measured lutropin and follitropin in blood and urine with radioimmunoassay kits from Diagnostic Products Corporation and compared the results with those obtained by use of reagents from the National Institutes of Health (NIH) and the World Health Organization (WHO). The urine standard (second IRP-HMG) from WHO, the blood standard (LER-907) from NIH, and the commercial standards all effected similar displacement of trace material when the commercial gonadotropin kit reagents were used. Highly significant correlations were achieved for these hormones in blood or urine on comparing commercial and NIH/WHO reagents. Serial dilutions of urine samples produced similar relative potencies with the commercial reagents. Conversion factors are presented to relate results for LER-907, second IRP, or commercial standards. Commercially available reagents can provide a practical and reliable means of gonadotropin radioimmunoassay in blood or urine.


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Copyright © 1981 by the American Association for Clinical Chemistry.