Clinical Chemistry
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Clinical Chemistry 27: 1896-1898, 1981;
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Clinical Chemistry, Vol 27, 1896-1898, Copyright © 1981 by American Association for Clinical Chemistry

Sample viscosity can be a source of analytical error when discrete sampler-dilutors are used

KM Chan and JH Ladenson

Total protein concentration in the serum of a patient with hyperviscosity syndrome differed as measured by the biuret procedure in the DuPont aca (80 g/L) and the SMA 12/60 (105 g/L), owing to viscosity- dependent errors with the aca sampling system; the magnitude depended on sample temperature and volume of sample aspirated. This kind of error was not observed with the SMA 12/60 and was far less severe when a Micromedic sampler-dilutor was tested. It could be eliminated in the case of the aca by adding sample to test packs with a syringe rather than with the aca automated sampler-dilutor. We thus recommend use of the syringe method when unusually viscous samples (serum or other body fluids) are analyzed in the aca.




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Copyright © 1981 by the American Association for Clinical Chemistry.