Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 27: 1922-1925, 1981;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kasahara, Y.
Right arrow Articles by Ashihara, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kasahara, Y.
Right arrow Articles by Ashihara, Y.

Clinical Chemistry, Vol 27, 1922-1925, Copyright © 1981 by American Association for Clinical Chemistry

Colorimetry of angiotensin-I converting enzyme activity in serum

Y Kasahara and Y Ashihara

This simple, accurate, and reproducible colorimetric method for determining the activity of angiotensin-I converting enzyme is based upon colorimetry of the quinoneimine dye produced from the substrate p- hydroxyhippuryl-L-histidyl-L-leucine by action of this enzyme through the following series of reactions. The enzyme acts on the substrate to yield p-hydroxyhippuric acid and L-histidyl-L-leucine. The former is then hydrolyzed in the presence of hippuricase to produce p- hydroxybenzoic acid and glycine. Finally, oxidative coupling of p- hydroxybenzoic acid with 4-aminoantipyrine is catalyzed by peroxidase in the presence of hydrogen peroxide, producing a quinoneimine dye, the concentration of which is measured at its absorbance maximum at 505 nm to evaluate the activity of ACE. The Km value for the above-mentioned substrate is 0.32 mmol/L, the optimum pH is 8.3. Results by the present method and Cushman and Cheung's method (Biochem. Pharmacol. 20: 1637, 1971) correlate closely (r = 0.986). The within-run CV is 2.93%.


The following articles in journals at HighWire Press have cited this article:


Home page
 Biol. Reprod.Home page
E. Deguchi, T. Tani, H. Watanabe, S. Yamada, and G. Kondoh
Dipeptidase-Inactivated tACE Action In Vivo: Selective Inhibition of Sperm-Zona Pellucida Binding in the Mouse
Biol Reprod, November 1, 2007; 77(5): 794 - 802.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Wakahara, T. Konoshita, S. Mizuno, M. Motomura, C. Aoyama, Y. Makino, N. Kato, I. Koni, and I. Miyamori
Synergistic Expression of Angiotensin-Converting Enzyme (ACE) and ACE2 in Human Renal Tissue and Confounding Effects of Hypertension on the ACE to ACE2 Ratio
Endocrinology, May 1, 2007; 148(5): 2453 - 2457.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
T. Handa, S. Nagai, Y. Fushimi, S. Miki, K. Ohta, A. Niimi, M. Mishima, and T. Izumi
Clinical and Radiographic Indices Associated With Airflow Limitation in Patients With Sarcoidosis
Chest, December 1, 2006; 130(6): 1851 - 1856.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
M. He, T. Ohrui, M. Maruyama, N. Tomita, K. Nakayama, M. Higuchi, K. Furukawa, and H. Arai
ACE activity in CSF of patients with mild cognitive impairment and Alzheimer disease.
Neurology, October 10, 2006; 67(7): 1309 - 1310.
[Full Text] [PDF]

Home page
 ChestHome page
T. Handa, S. Nagai, S. Miki, Y. Fushimi, K. Ohta, M. Mishima, and T. Izumi
Incidence of Pulmonary Hypertension and Its Clinical Relevance in Patients With Sarcoidosis
Chest, May 1, 2006; 129(5): 1246 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Nozawa, K.-i. Sugimoto, M. Ohmori, H. Ando, and A. Fujimura
Dosing Time-Dependent Effect of Temocapril on the Mortality of Stroke-Prone Spontaneously Hypertensive Rats
J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 176 - 181.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H. Senzaki, C.-H. Chen, H. Ishido, S. Masutani, T. Matsunaga, M. Taketazu, T. Kobayashi, N. Sasaki, S. Kyo, and Y. Yokote
Arterial Hemodynamics in Patients After Kawasaki Disease
Circulation, April 26, 2005; 111(16): 2119 - 2125.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
F A Sayed-Tabatabaei, A F C Schut, A Hofman, A M Bertoli-Avella, J Vergeer, J C M Witteman, and C M van Duijn
A study of gene-environment interaction on the gene for angiotensin converting enzyme: a combined functional and population based approach
J. Med. Genet., February 1, 2004; 41(2): 99 - 103.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. G. Boot, M. Verhoek, M. de Fost, C. E. M. Hollak, M. Maas, B. Bleijlevens, M. J. van Breemen, M. van Meurs, L. A. Boven, J. D. Laman, et al.
Marked elevation of the chemokine CCL18/PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention
Blood, January 1, 2004; 103(1): 33 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
M. Kitakaze, H. Asanuma, H. Funaya, K. Node, S. Takashima, S. Sanada, M. Asakura, H. Ogita, J. Kim, and M. Hori
Angiotensin-converting enzymeinhibitors and angiotensin iireceptor blockers synergistically increasecoronary blood flow in canine ischemic myocardium: Role of bradykinin
J. Am. Coll. Cardiol., July 3, 2002; 40(1): 162 - 166.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
N. Yamamoto, H. Yasue, Y. Mizuno, M. Yoshimura, H. Fujii, M. Nakayama, E. Harada, S. Nakamura, T. Ito, and H. Ogawa
Aldosterone Is Produced From Ventricles in Patients With Essential Hypertension
Hypertension, May 1, 2002; 39(5): 958 - 962.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Ito, A. Itakura, Y. Ohno, M. Nomura, T. Senga, T. Nagasaka, and S. Mizutani
Possible Activation of the Renin-Angiotensin System in the Feto-Placental Unit in Preeclampsia
J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1871 - 1878.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
H Funatsu, H Yamashita, Y Nakanishi, and S Hori
Angiotensin II and vascular endothelial growth factor in the vitreous fluid of patients with proliferative diabetic retinopathy
Br. J. Ophthalmol., March 1, 2002; 86(3): 311 - 315.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Hu, A. Igarashi, M. Kamata, and H. Nakagawa
Angiotensin-converting Enzyme Degrades Alzheimer Amyloid beta -Peptide (Abeta ); Retards Abeta Aggregation, Deposition, Fibril Formation; and Inhibits Cytotoxicity
J. Biol. Chem., December 14, 2001; 276(51): 47863 - 47868.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Takeshita, H. Tomiyama, N. Yokoyama, Y. Kawamura, T. Furukawa, Y. Ishigai, T. Shibano, T. Isshiki, and T. Sato
Angiotensin-converting enzyme inhibition improves defective angiogenesis in the ischemic limb of spontaneously hypertensive rats
Cardiovasc Res, November 1, 2001; 52(2): 314 - 320.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. Kramers, S. M. Danilov, J. Deinum, I. V. Balyasnikova, N. Scharenborg, M. Looman, F. Boomsma, M. H. de Keijzer, C. van Duijn, S. Martin, et al.
Point Mutation in the Stalk of Angiotensin-Converting Enzyme Causes a Dramatic Increase in Serum Angiotensin-Converting Enzyme But No Cardiovascular Disease
Circulation, September 11, 2001; 104(11): 1236 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Hermans, M. Petrek, V. Kolek, B. Weynand, T. Pieters, M. Lambert, and A. Bernard
Serum Clara cell protein (CC16), a marker of the integrity of the air-blood barrier in sarcoidosis
Eur. Respir. J., September 1, 2001; 18(3): 507 - 514.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Y. Mizuno, M. Yoshimura, H. Yasue, T. Sakamoto, H. Ogawa, K. Kugiyama, E. Harada, M. Nakayama, S. Nakamura, T. Ito, et al.
Aldosterone Production Is Activated in Failing Ventricle in Humans
Circulation, January 2, 2001; 103(1): 72 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child.Home page
T. Yoshioka, Y.-x. Xu, H. Yoshida, H. Shiraga, T. Muraki, and K. Ito
Deletion polymorphism of the angiotensin converting enzyme gene predicts persistent proteinuria in Henoch-Schonlein purpura nephritis
Arch. Dis. Child., November 1, 1998; 79(5): 394 - 399.
[Abstract] [Full Text]

Home page
 HypertensionHome page
Y. Higashi, T. Oshima, S. Sasaki, Y. Nakano, M. Kambe, H. Matsuura, and G. Kajiyama
Angiotensin-Converting Enzyme Inhibition, But Not Calcium Antagonism, Improves a Response of the Renal Vasculature to L-Arginine in Patients With Essential Hypertension
Hypertension, July 1, 1998; 32(1): 16 - 24.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
Y. Takemoto, M. Sakatani, S. Takami, T. Tachibana, J. Higaki, T. Ogihara, T. Miki, T. Katsuya, T. Tsuchiyama, A. Yoshida, et al.
Association between angiotensin II receptor gene polymorphism and serum angiotensin converting enzyme (SACE) activity in patients with sarcoidosis
Thorax, June 1, 1998; 53(6): 459 - 462.
[Abstract] [Full Text]

Home page
 HypertensionHome page
Y. Watanabe, T. Ishigami, Y. Kawano, T. Umahara, A. Nakamori, S. Mizushima, K. Hibi, I. Kobayashi, K. Tamura, H. Ochiai, et al.
Angiotensin-Converting Enzyme Gene I/D Polymorphism and Carotid Plaques in Japanese
Hypertension, September 1, 1997; 30(3): 569 - 573.
[Abstract] [Full Text]

Home page
 HypertensionHome page
K. Hibi, T. Ishigami, K. Kimura, M. Nakao, T. Iwamoto, K. Tamura, T. Nemoto, T. Shimizu, Y. Mochida, H. Ochiai, et al.
Angiotensin-Converting Enzyme Gene Polymorphism Adds Risk for the Severity of Coronary Atherosclerosis in Smokers
Hypertension, September 1, 1997; 30(3): 574 - 579.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. TOMITA, Y. INA, Y. SUGIURA, S. SATO, H. KAWAGUCHI, M. MORISHITA, M. YAMAMOTO, and R. UEDA
Polymorphism in the Angiotensin-Converting Enzyme (ACE) Gene and Sarcoidosis
Am. J. Respir. Crit. Care Med., July 1, 1997; 156(1): 255 - 259.
[Abstract] [Full Text]

Home page
 CirculationHome page
M. Hosoi, Y. Nishizawa, K. Kogawa, T. Kawagishi, T. Konishi, K. Maekawa, M. Emoto, S. Fukumoto, A. Shioi, T. Shoji, et al.
Angiotensin-Converting Enzyme Gene Polymorphism Is Associated With Carotid Arterial Wall Thickness in Non–Insulin-Dependent Diabetic Patients
Circulation, August 15, 1996; 94(4): 704 - 707.
[Abstract] [Full Text]

Home page
 CirculationHome page
M. Kitakaze, T. Minamino, K. Node, K. Komamura, Y. Shinozaki, H. Mori, H. Kosaka, M. Inoue, M. Hori, and T. Kamada
Beneficial Effects of Inhibition of Angiotensin-Converting Enzyme on Ischemic Myocardium During Coronary Hypoperfusion in Dogs
Circulation, August 15, 1995; 92(4): 950 - 961.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
M. Sakaki, Y. Taenaka, E. Tatsumi, T. Nakatani, and H. Takano
Influences of nonpulsatile pulmonary flow on pulmonary functionEvaluation in a chronic animal model
J. Thorac. Cardiovasc. Surg., September 1, 1994; 108(3): 495 - 502.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Association for Clinical Chemistry.