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Clinical Chemistry 27: 1993-1996, 1981;
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Clinical Chemistry, Vol 27, 1993-1996, Copyright © 1981 by American Association for Clinical Chemistry

Effect of variations in globulin concentrations on serum radioimmunoassay results, as exemplified by choriogonadotropin

MJ Jawad and EA Wilson

Variations in serum protein concentrations can affect results of radioimmunoassay of human choriogonadotropin involving either polyethylene glycol or double-antibody methods of separation. Relative to the maximal binding of choriogonadotropin in the presence of normal (30 g/L) globulin concentrations, globulin concentrations of 10 to 60 g/L were associated with maximal binding ranging from 84.9 to 115.6% in the polyethylene glycol system, and from 109.9 to 92.5% in the double- antibody system. This effect was quantitatively much the same throughout the standard curve in the polyethylene glycol system, but diminished with the higher standard concentrations in the double- antibody system. In assays involving polyethylene glycol separation, an increase in globulin was associated with a linear increase in nonspecific binding, but no detectable effect of globulin on nonspecific binding was observed in the double-antibody system. Determination and compensation for nonspecific binding of individual samples (rather than for mean assay nonspecific binding) eliminated the globulin effect in the polyethylene glycol system but not in the double- antibody system.




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