Clinical Chemistry
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Clinical Chemistry 27: 2035-2037, 1981;
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Clinical Chemistry, Vol 27, 2035-2037, Copyright © 1981 by American Association for Clinical Chemistry

A coupled NAD+-peroxidase spectrophotometric assay for cholesterol

G Avigad and B Robertson

We describe a modified enzymic reagent for determination of cholesterol and cholesterol ester in serum and in high-density lipoprotein. In this new procedure, the hydrogen peroxide produced by the action of cholesterol oxidase is used as a substrate for NAD+ peroxidase. Spectrophotometric determination of the NADH consumed in this coupled reaction provides a direct and absolute measure of the cholesterol originally present in free form and that liberated by the action of cholesterol ester hydrolase.




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Copyright © 1981 by the American Association for Clinical Chemistry.