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Clinical Chemistry 27: 263-267, 1981;
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Clinical Chemistry, Vol 27, 263-267, Copyright © 1981 by American Association for Clinical Chemistry

Radioassay for cobalamin (vitamin B12) requiring no pretreatment of serum

TM Houts and JA Carney

We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is released from its binding proteins, whereas the binding agent maintains a high affinity for cobalamin (Ka 1.7 x 10(10) L/mol). Under these assay conditions the binding protein shows a specificity for cobalamin similar to that of purified intrinsic factor. The assay is simple, rapid, and precise, and results correlate well with those of the Euglena gracilis microbiological assay and an intrinsic-factor binding assay.




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Copyright © 1981 by the American Association for Clinical Chemistry.