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Clinical Chemistry 27: 268-271, 1981;
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Clinical Chemistry, Vol 27, 268-271, Copyright © 1981 by American Association for Clinical Chemistry

Ultramicro determination of serum triglycerides by bioluminescent assay

M Werner, DG Gabrielson and J Eastman

The ultramicro (1-microL samples) assay of serum or plasma triglycerides that we describe here is potentially applicable to 1-nL samples and to isolated cells. This technically simple method involves only three reactions: (a) enzymic hydrolysis with lipase and alpha- chymotrypsin; (b) conversion by glycerol kinase of the liberated glycerol and of adenosine triphosphate added in excess to glycerol-1- phosphate and to adenosine diphosphate; and (c) assay of the residual adenosine triphosphate by the luciferin-luciferase reaction. The assay was optimized with respect to glycerol kinase, buffer, pH, temperature, and adenosine triphosphate. Performance characteristics compare well with those of traditional triglyceride assays.




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Copyright © 1981 by the American Association for Clinical Chemistry.