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Clinical Chemistry 27: 310-313, 1981;
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Clinical Chemistry, Vol 27, 310-313, Copyright © 1981 by American Association for Clinical Chemistry

Nephelometry of human IgG subclass concentration in serum

OE Beck and PE Kaiser

An immunoassay method for determination of human IgG subclass concentration by rate nephelometry was developed by using the Beckman Immunochemistry Analyzer, subclass-specific antisera, and a human serum standard. Twelve sera derived from normal as well as gammapathological patients were analyzed. The total IgG concentration, as determined by using a readily available kit, correlated with the sum of the concentration of the individual subclasses. Most of the gammapathological cases were shown to be the IgG1 subclass, which was confirmed by radial immunodiffusion.




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Copyright © 1981 by the American Association for Clinical Chemistry.