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Clinical Chemistry 27: 388-390, 1981;
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Clinical Chemistry, Vol 27, 388-390, Copyright © 1981 by American Association for Clinical Chemistry

Fluorescent spot test for detecting carbohydrate-splitting enzymes in meconium

MC Hsieh

I describe a fluorescent spot test for detecting the acidic glycosidases in meconium. Meconium is mixed with various 4- methylumbelliferylglycosides at the appropriate pH. In the presence of carbohydrate-splitting enzymes, 4-methylumbelliferone, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence for specimens containing acidic glycosidases. The technique is applicable to any test that involves production of fluorescent products from non-fluorescent reactants. The procedure was applied to meconium specimens selected because of increased albumin content known to be associated with cystic fibrosis in newborns. The presence of beta-D-fucosidase in meconium was correlated to increased albumin content. Detection of beta-D-fucosidase by the present procedure may be useful as a screening test for cystic fibrosis.




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Copyright © 1981 by the American Association for Clinical Chemistry.