Clinical Chemistry
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Clinical Chemistry 27: 397-401, 1981;
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Clinical Chemistry, Vol 27, 397-401, Copyright © 1981 by American Association for Clinical Chemistry

Liquid-chromatographic analysis for urinary porphyrins

RE Ford, CN Ou and RD Ellefson

Urinary porphyrins are separated, according to number of carboxyl groups, in a system consisting of a mu-Bondapak C18 stationary phase and a mobile phase of methanol and aqueous sodium phosphate (pH 3.5) in a linear gradient. The specimen is prepared simply by adjusting the pH of a 5-mL sample to 2.0 and removing solids by centrifugation. The eluted porphyrins are measured fluorometrically. Naturally occurring non-porphyrin fluorescent substances are eluted ahead of the porphyrins. Chromatography requires about 20 min, and a re- establishment of initial conditions requires an additional 15 min.


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Copyright © 1981 by the American Association for Clinical Chemistry.