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Clinical Chemistry 27: 422-426, 1981;
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Clinical Chemistry, Vol 27, 422-426, Copyright © 1981 by American Association for Clinical Chemistry

Improved determination of D-glucaric acid in urine

K Jung, D Scholz and G Schreiber

Using a simplex optimization procedure, we have elaborated a sensitive and reliable micromethod for determining D-glucaric acid, based on inhibition of glucuronidase activity by 1,4-D-glucarolactone, produced from D-glucaric acid by heating. The maximum attainable difference between glucuronidase activity in the reaction mixture with and without D-glucaric acid was used as an optimization criterion. From the optimization scheme, 1 mmol/L 4-nitrophenyl-beta-D-glucuronide and pH 5.0 were found to be optimal conditions for determination of D-glucaric acid with this test. In contrast to other similar methods, in which a logarithmic calibration curve is used, we calculate D-glucaric acid with a linear calibration curve by using the Dixon (Biochem. J. 55: 170, 1953) plot. The coefficient of variation for the method (within- run and between-day precision) was from 3.1 to 5.8%. Men excrete more D- glucaric acid than do women per 24 hours, but we saw no sex-related differences in excretion of D-glucaric acid when it was expressed in terms of urinary creatinine.




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Copyright © 1981 by the American Association for Clinical Chemistry.