Clinical Chemistry
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Clinical Chemistry 27: 441-443, 1981;
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Clinical Chemistry, Vol 27, 441-443, Copyright © 1981 by American Association for Clinical Chemistry

Fluorometric liquid-chromatographic determination of serum cortisol

GR Gotelli, JH Wall, PM Kabra and LJ Marton

We describe a sensitive and specific liquid-chromatographic assay for serum cortisol, exploiting acid-induced fluorescence. Each analysis requires only 50 microL of serum, and chromatography is complete in 7 min. Analytical recovery of cortisol added to serum ranged from 90 to 105% and between-run precision (CV) from 6 to 8%. The lower limit of detection for cortisol is 10 micrograms/L, and linearity extends to 1600 micrograms/L. Numerous steroids and drugs tested did not interfere.


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Copyright © 1981 by the American Association for Clinical Chemistry.