Clinical Chemistry
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Clinical Chemistry 27: 464-465, 1981;
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Clinical Chemistry, Vol 27, 464-465, Copyright © 1981 by American Association for Clinical Chemistry

Colorimetric determination of serum 5'-nucleotidase without deproteinization

RJ Wood and DG Williams

We present a sensitive colorimetric determination of 5'-nucleotidase based on the measurement of liberated phosphate by reaction with stannous chloride/molybdenum blue. No protein precipitation is required. The method is simple, sensitive, economical, and easy to use, and involves relatively stable reagents.




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Copyright © 1981 by the American Association for Clinical Chemistry.